SchauerLab

Publications

Preprints and/or in preparation

  • Timmerman AJ, Brady RE, Lawson VM, Stewart JA, Argueso JL, Schauer GD. Mechanical tuning of replication stress tolerance and genomic stability through the checkpoint mediator Mrc1. bioRxiv: The Preprint Server for Biology; 2026. doi:10.64898/2026.05.14.725212 PMCID: PMC13192788
  • Reitman HJ, Uhrig ME, Karns CR, Shaw AE, Mihelich MN, Wiese C, Schauer GD. Hydroxyurea-induced cell cycle arrest in human cells is not caused by dNTP depletion. Manuscript in preparation.
  • Dixon LD, Radebaugh CA, Schauer GD, Stargel LA. Spn1 and its network of interactions in the RNA Polymerase II complex. Manuscript in Preparation

Schauer Lab publications

  • Mukherjee, P, Sarker, A, Schauer, GD  DNA Polymerase α has cyclopyrimidine dimer translesion activity that is suppressed during normal replication. In press at Journal of Molecular Biology, September 2026.
  • Mukerhjee, P and Schauer, GD. A simplified translesion polymerase assay. Invited book chapter, Methods in Molecular Biology (Springer), 2026.
  • Shaw AE, Mihelich MN, Whitted JE, Reitman HJ, Timmerman AJ, Tehseen M, Hamdan SM, Schauer GD. Revised mechanism of hydroxyurea-induced cell cycle arrest and an improved alternative. Proc Natl Acad Sci USA. 2024 Oct 15;121(42):e2404470121.
  • Shaw AE, Kairamkonda S, Ghodke H, Schauer GD. Biochemical and single-molecule techniques to study accessory helicase resolution of R-loop proteins at stalled replication forks. Methods Enzymol. April 2022; 673:191-225.  
  • Stewart, JA, Schauer GD, Bhagwat AS. Visualization of uracils created by APOBEC3A using UdgX shows colocalization with RPA at stalled replication forks. Nucleic Acids Research, 2020 Oct. 19;48(20):e118.
  • Schauer GD†*, Spenkelink LM†, Lewis JS, Yurieva O, Mueller SH, van Oijen AM*, O’Donnell ME*, Replisome bypass of a protein-based R-loop block by Pif1. Proc Natl Acad Sci USA, 2020 Dec. 1; 117(48):30354–30361. †co-first author; *corresponding author.

Previous work

  • Yuan Z, Georgescu R, Schauer GD, O’Donnell ME, Li H. Structure of the polymerase ε holoenzyme and atomic model of the leading strand replisome. Nat Commun.; 2020 Jun 22;11(1):3156.
  • Lewis JS†, Spenkelink LM†, Schauer GD, Yurieva O, Natarajan V, Kaur G, Maher C, Kay C, O’Donnell ME, van Oijen AM. Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication. Molecular Cell, 2020 Jan 2; 77, 17-25; †co-first author
  • Schauer GD†, Wasserman MR†, O’Donnell ME*, Liu S*.  Replisome preservation by a single-stranded DNA gate in the CMG helicase. Cell, 2019 Jul 25;178(3):600-611.e16. †co-first author; *co-corresponding author
  • Whinn K,Kaur G, Lewis, JS, Schauer GD, Müller S, Jergic S, Naganbabu M, Bruchez M, O’Donnell ME, Dixon N, van Oijen AM, Ghodke H. Nuclease dead Cas9 is a programmable roadblock for DNA replication.  Sci Rep. 2019 Sep 16;9(1):13292.
  • Lewis JS†, Spenkelink LM†, Schauer GD, Hill FR, Georgescu RE, O’Donnell ME, van Oijen AM. Single-molecule visualization of Saccharomyces cerevisiae leading-strand synthesis reveals dynamic interaction between MTC and the replisome. Proc Natl Acad Sci USA. 2017 Oct 3;114(40):10630–10635. PMCID: PMC5635917; †authors contributed equally.
  • Schauer GD, Finkelstein J, O’Donnell M. In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication. Bio-Protocol. 2017 Sep 20;7(18). PMCID: PMC5659624
  • Langston LD, Mayle R, Schauer GD, Yurieva O, Zhang D, Yao NY, Georgescu RE, O’Donnell ME. Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks. eLife. 2017 Sep 4;6. PMCID: PMC5599239
  • Schauer GD, O’Donnell ME. Quality control mechanisms exclude incorrect polymerases from the eukaryotic replication fork. Proc Natl Acad Sci USA. 2017 Jan 9. PMCID: PMC5278475
  • Georgescu RE, Schauer GD, Yao NY, Langston LD, Yurieva O, Zhang D, Finkelstein J, O’Donnell ME. Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operation. eLife. 2015 Apr 30. PMCID: PMC4413876.
  • Schauer GD, and Sluis-Cremer N. HIV-1 Resistance to Reverse Transcriptase Inhibitors. Handbook of Antimicrobial Resistance, M. Gotte, A. Berghuis, G. Matlashewski, M. Wainberg, and D. Sheppard, eds. (Springer).  2014; 1–17. DOI: 10.1007/978-1-4939-0667-3_26-1
  • Schauer GD, Leuba S, Sluis-Cremer N. Biophysical Insights into the Inhibitory Mechanism of Non-Nucleoside HIV-1 Reverse Transcriptase Inhibitors. Biomolecules. 2013;3(4):889-904.
  • Schauer GD, Huber KD, Leuba SH, Sluis-Cremer N. Mechanism of allosteric inhibition of HIV-1 reverse transcriptase revealed by single-molecule and ensemble fluorescence. Nucleic Acids Research. 2014 Oct;42(18):11687–11696. PMCID: PMC4191400.
  • Leuba SH, Carney SM, Dahlburg EM, Eells RJ, Ghodke H, Yanamala N, Schauer GD, Klein-Seetharaman J. Early integration of the individual student in academic activities: a novel classroom concept for graduate education in molecular biophysics and structural biology. BMC Biophysics. 2014;7:6. PMCID: PMC4134111.
  • Fagerburg MV, Schauer GD, Thickman KR, Bianco PR, Khan SA, Leuba SH, Anand SP. PcrA-mediated disruption of RecA nucleoprotein filaments–essential role of the ATPase activity of RecA. Nucleic Acids Research. 2012;40(17):8416-24. PMCID: PMC3458574.
  • Graham BW, Schauer GD, Leuba SH, Trakselis MA. Steric exclusion and wrapping of the excluded DNA strand occurs along discrete external binding paths during MCM helicase unwinding. Nucleic Acids Research. 2011;39(15):6585-95. PMCID: PMC3159478.